mouse monoclonal anti-epha4 receptor (Thermo Fisher)

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Thermo Fisher mouse monoclonal anti-epha4 receptor

(A) Representative images of growth cones from control and RhoA cKO cortical neurons after a 30 min incubation with preclustered ephrinB3-Fc or Fc alone. Scale bar:10 µm. (B) Quantification of the growth cone collapse response of control and RhoA cKO neurons treated with ephrinB3-Fc or Fc (*p<0.001, ANOVA followed by the Tukey test). (C) Cortical neurons from RhoA cKO mice express <t>EphA4</t> at levels similar to control neurons. (D) Percentage of ephrinB3-induced growth cone collapse in rat cortical neurons pretreated or not with the ROCK inhibitor Y-27632 (*p<0.001, ANOVA followed by the Tukey test). (E) Expression of ephrinB3 is lost from the midline at L2 in RhoA cKO mice. Arrowheads show ephrinB3 positive staining in the spinal cord of P5 mice. Scale bar: 10 µm. Three sections per animal and N = 3 animals were analyzed per genotype.

Mouse Monoclonal Anti Epha4 Receptor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-epha4/pmc03692541-32-21-29
Average 90 stars, based on 1 article reviews

mouse monoclonal anti-epha4 receptor - by Bioz Stars, 2026-09

90/100 stars

Images

1) Product Images from "The Small GTPase RhoA Is Required for Proper Locomotor Circuit Assembly"

Article Title: The Small GTPase RhoA Is Required for Proper Locomotor Circuit Assembly

Journal: PLoS ONE

doi: 10.1371/journal.pone.0067015

Figure Legend Snippet: (A) Representative images of growth cones from control and RhoA cKO cortical neurons after a 30 min incubation with preclustered ephrinB3-Fc or Fc alone. Scale bar:10 µm. (B) Quantification of the growth cone collapse response of control and RhoA cKO neurons treated with ephrinB3-Fc or Fc (*p<0.001, ANOVA followed by the Tukey test). (C) Cortical neurons from RhoA cKO mice express EphA4 at levels similar to control neurons. (D) Percentage of ephrinB3-induced growth cone collapse in rat cortical neurons pretreated or not with the ROCK inhibitor Y-27632 (*p<0.001, ANOVA followed by the Tukey test). (E) Expression of ephrinB3 is lost from the midline at L2 in RhoA cKO mice. Arrowheads show ephrinB3 positive staining in the spinal cord of P5 mice. Scale bar: 10 µm. Three sections per animal and N = 3 animals were analyzed per genotype.

Techniques Used: Incubation, Expressing, Staining

In control mice, when EphA4-expressing CST and spinal interneuron (IN) axons encounter ephrinB3 at the spinal cord midline, they are repulsed due to the initiation of signaling pathways that involve RhoA activation and Rac1 inhibition. Loss of RhoA causes aberrant midline crossing of CST and spinal IN axons due to a failure of neurons to retract their axons and/or the absence of ephrinB3 expression at the midline.

Figure Legend Snippet: In control mice, when EphA4-expressing CST and spinal interneuron (IN) axons encounter ephrinB3 at the spinal cord midline, they are repulsed due to the initiation of signaling pathways that involve RhoA activation and Rac1 inhibition. Loss of RhoA causes aberrant midline crossing of CST and spinal IN axons due to a failure of neurons to retract their axons and/or the absence of ephrinB3 expression at the midline.

Techniques Used: Expressing, Activation Assay, Inhibition

Western Blot:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: The following primary antibodies were used: mouse anti-HA (cat. no. 11583816001, Roche), mouse anti-Flag M2 (cat. no. F3165, clone M2; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-myc derived from hybridoma MYC1-9E10.2 (cat. no. CRL-1729, ATCC), mouse anti-phosphotyrosine (clone 4G10, cat. no. 05–321, Millipore, Kankakee, IL, USA), and mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA); mouse anti-EphA4 (cat. no. sc-365503), rabbit anti-PDGFRβ (cat. no. sc-432), mouse anti-PDGFRβ (cat. no. sc-374573), rabbit anti-FGFR1 (cat. no. sc-121), mouse anti-GFP (cat. no. sc-9996), rabbit anti-GAPDH (cat. no. sc-25778), and rabbit anti-FRS2a (cat. no. sc-8318) were purchased from Santa Cruz Biotechnology, Santa Cruz, CA, USA; rabbit anti-phosph-p44/42 mitogen activated protein kinase (MAPK; ERK; Thr202/Tyr204; cat. no. 9101), rabbit anti-p44/42 MAPK (ERK; cat. no. 9102), and rabbit anti-FRS2α (Y196; cat. no. 3861) were purchased from Cell Signaling Technology, Danvers, MA, USA.

Article Title: Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice
Article Snippet: For primary antibodies, we used rabbit anti-Nnat (1:1000, Abcam, catalog ab27266), mouse anti-SLC4A8/SLC4A10 (1:1000, Invitrogen, catalog PA5-101904), mouse anti-EphA4 (1:1000, Invitrogen, catalog 37-1600), mouse anti-BACE1 (1:1000, Thermo Fisher, catalog MA1-177), rabbit anti-BCL6 (1:1000, Invitrogen, catalog PA5-27390), mouse anti HAP1 (1:1000, Invitrogen, catalog MA1-46412), mouse anti-GAPDH (1:3000, Invitrogen, catalog MA5-15738-D680), rabbit anti-BiP/GRP78 (1:1000, ABclonal, catalog AB2757054), rabbit anti-PSD95 (1:1000, Cell Signaling Technology, catalog 3450), rabbit anti-CaMKII (1:1000, Cell Signaling Technology, catalog 3362), and rabbit anti-phospho-CaMKII (1:1000, Cell Signaling Technology, catalog 12716).

Article Title: Impaired Spatial Memory in Mice Lacking CD3ζ Is Associated with Altered NMDA and AMPA Receptors Signaling Independent of T-Cell Deficiency
Article Snippet: The primary antibodies used in this study were as follows: mouse anti-EphA4 (1 μg/ml; Invitrogen), rabbit anti-CD3ζ (0.5 μg/ml; Spring Bioscience), mouse anti-PSD95 (1 μg/ml; Abcam), mouse anti-GluN1 (1 μg/ml; BD Pharmingen), rabbit anti-GluN2A (2 μg/ml; Millipore), mouse anti-GluN2B (2 μg/ml; NeuroMab), rabbit anti-GluA1 (5 μg/ml; Calbiochem), rabbit anti-Phospho831 GluA1 (Millipore), rabbit anti-Phospho286 CaMKII (1:200; Promega), mouse anti-CaMKIIα (0.5 μg/ml; Thermo Scientific), or mouse anti-GAPDH (0.2 μg/ml; Santa Cruz Biotechnologies).

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Article Title: Plasmin-mediated cleavage of EphA4 at central amygdala inhibitory synapses controls anxiety
Article Snippet: Samples were then probed with the following primary antibodies: mouse anti-EphA4, (Invitrogen, USA, 1:500), anti-GFP antibody (Santa Cruz Biotechnology Inc., USA, 1:500) at 4°C overnight with gentle agitation.

Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
Article Snippet: Tissue sections were incubated overnight at 4°C with primary antibodies prepared in blocking solution using the following concentrations: goat anti-doublecortin (Dcx) 1:200 (Santa Cruz Biotechnology, RRID: AB_2088494), guinea pig anti-Dcx 1:1000 (Millipore, RRID: AB_1586992), rabbit anti-Dcx 1:200 (Santa Cruz Biotechnology, AB_2088488), rabbit anti-GFAP 1:1000 (DAKO, RRID: AB_10013382), mouse anti-GFAP 1:400 (Millipore, RRID: AB_2109815), rat anti-GFAP clone 2.2B10 1:250 (Thermo Fisher Scientific, RRID: AB_86543), goat antiPECAM-1 1:50 (Santa Cruz Biotechnology, RRID: 632174), rabbit antiEphA4 1:50 (Abcam, RRID: AB_304857), rat anti-EphA4 (Abcam, RRID: AB_2099346), mouse anti-EphA4 1:50 (Thermo Fisher Scientific, RRID: AB_2533301), goat anti-EphA4 1:20 (R&D Systems, AB_2099371), rabbit anti-ephrinA2 1:50 (Santa Cruz Biotechnology, RRID: AB_631409), rabbit anti-ephrinA3 1:50 (Santa Cruz Biotechnology, RRID: AB_2097777), rabbit anti-ephrinA5 1:50 (Santa Cruz Biotechnology, RRID: AB_2097911), or rabbit anti-ephrinB1/2/3 1:50 (Santa Cruz Biotechnology, RRID: AB_631413).

Article Title: Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve
Article Snippet: Primary antibodies were rabbit anti-Nogo-A/B (Bianca, Rb140) serum (1 : 20 000), mouse anti-L-MAG serum (1 : 1 000, polyclonal), 59 mouse anti-EphA4 (1 : 200, Invitrogen, Zug, Switzerland), rabbit anti-EphrinA3 (1 : 200, Invitrogen), mouse anti-S1PR2 (1 : 500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-phospho-STAT3 (1 : 500, Cell Signaling), rabbit anti-STAT3 (1 : 500, Cell Signaling), rabbit anti-phospho-ERK1/2 (1 : 1000, Cell Signaling), rabbit anti-ERK1/2 (1 : 1000, Cell Signaling), rabbit anti-phospho-AKT (1 : 500, Cell Signaling), rabbit anti-AKT (1 : 500, Cell Signaling), rabbit anti-phospho-CREB (1 : 500, Cell Signaling), rabbit anti-CREB (1 : 500, Cell Signaling), rabbit anti-phospho-Cofilin (1 : 500, Abcam), rabbit anti-Cofilin (1 : 500, Cell Signaling), and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1 : 20 000; Abcam).

Article Title: EphA2 is a functional receptor for the growth factor progranulin
Article Snippet: SuperSignal West Pico Enhanced Chemiluminescence substrate and mouse anti-EphA4 were purchased from Thermo Fisher Scientific.

Purification:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Recombinant:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Mass Spectrometry:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Immunoprecipitation:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Binding Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Expressing:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Variant Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Over Expression:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Transfection:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Plasmid Preparation:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Construct:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Injection:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Immunofluorescence:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Incubation:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Derivative Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Cell Culture:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Labeling:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Software:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Dominant Negative Mutation:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Mutagenesis:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Activation Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Standard Deviation:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

BrdU Incorporation Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Immunostaining:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

BrdU Staining:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Staining:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Fluorescence:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Microscopy:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Infection:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Cotransfection:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Blocking Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Activity Assay:

Article Title: The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo
Article Snippet: For immunofluorescence analysis, NPCs were fixed with 4% paraformaldehyde at room temperature for 1 h and blocked in 1% normal goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The cells were then incubated with mouse anti-Tuj1 antibody (cat. no. T8660; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-Nestin (cat. no. ab22035; Abcam, Cambridge, UK), rabbit anti-Ki67 (cat. no. ab16667, Abcam, Cambridge, UK), rabbit anti-GFAP (cat. no. Z0334, Dako), mouse anti-EphA4 (cat. no. 37–1,600, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-PDGFRβ (cat. no. sc-432) at 4°C overnight, followed by incubation with the secondary antibodies, goat anti-mouse Alexa Flour 488 (cat. no. 115-545-146; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) and goat anti-rabbit cyanine Cy3-conjugated IgG (cat. no. 711-165-152; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) for 2 h at room temperature in the dark.

Review